Correction of spherical surface measurements by confocal microscopy
نویسندگان
چکیده
منابع مشابه
Automated spherical aberration correction in scanning confocal microscopy.
Mismatch between the refractive indexes of immersion media and glass coverslips introduces spherical aberrations in microscopes especially for high numerical aperture objectives. This contribution demonstrates an automated adjustment of the coverslip correction collar in scanning confocal microscopy to compensate for spherical aberrations due to coverslip thickness mismatch. With a motorized co...
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Background To report normal variations of the limbal structures using in vivo laser scanning confocal microscopy. Methods: This was a retrospective study of fourteen eyes from 11 healthy individuals. Confocal imaging of cornea and limbus was performed using Heidelberg Retina Tomograph III Rostock Corneal Module. Results: The typical structure of the palisades of Vogt (POV) was detected ...
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Most confocal microscopes do not produce images in real time with nonlaser light sources. The tandem scanning confocal microscope does produce such images but, because the pinhole apertures of the Nipkov disk must be placed far apart to reduce cross talk between neighboring pinholes, only 1% or less of the light available for imaging is used. We show that, by using aperture correlation techniqu...
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The ocular globe is composed of three layers. The outer layer, known as the fibrous tunic, is further divided into the cornea and sclera. This layer gives shape to the eye, which is imperative for functioning of the visual system. The cornea is the most powerful refractory tissue of the eye [1]. Its surface location promotes continuous contact with infectious agents, and also predispose to inju...
متن کاملConfocal Microscopy
A confocal microscope creates sharp images of a specimen that would otherwise appear blurred when viewed with a conventional microscope. This is achieved by excluding most of the light from the specimen that is not from the microscope’s focal plane. The image has less haze and better contrast than that of a conventional microscope and represents a thin cross-section of the specimen. Thus, apart...
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ژورنال
عنوان ژورنال: Measurement Science and Technology
سال: 2020
ISSN: 0957-0233,1361-6501
DOI: 10.1088/1361-6501/ab786b